全文获取类型
收费全文 | 4868篇 |
免费 | 281篇 |
国内免费 | 530篇 |
专业分类
林业 | 529篇 |
农学 | 844篇 |
基础科学 | 5篇 |
251篇 | |
综合类 | 1724篇 |
农作物 | 569篇 |
水产渔业 | 213篇 |
畜牧兽医 | 974篇 |
园艺 | 467篇 |
植物保护 | 103篇 |
出版年
2024年 | 16篇 |
2023年 | 76篇 |
2022年 | 89篇 |
2021年 | 123篇 |
2020年 | 160篇 |
2019年 | 134篇 |
2018年 | 86篇 |
2017年 | 166篇 |
2016年 | 279篇 |
2015年 | 264篇 |
2014年 | 261篇 |
2013年 | 268篇 |
2012年 | 382篇 |
2011年 | 359篇 |
2010年 | 323篇 |
2009年 | 362篇 |
2008年 | 348篇 |
2007年 | 362篇 |
2006年 | 290篇 |
2005年 | 216篇 |
2004年 | 187篇 |
2003年 | 158篇 |
2002年 | 111篇 |
2001年 | 109篇 |
2000年 | 97篇 |
1999年 | 56篇 |
1998年 | 57篇 |
1997年 | 55篇 |
1996年 | 56篇 |
1995年 | 38篇 |
1994年 | 46篇 |
1993年 | 38篇 |
1992年 | 26篇 |
1991年 | 24篇 |
1990年 | 22篇 |
1989年 | 13篇 |
1988年 | 9篇 |
1987年 | 3篇 |
1986年 | 3篇 |
1985年 | 1篇 |
1981年 | 2篇 |
1978年 | 1篇 |
1963年 | 2篇 |
1955年 | 1篇 |
排序方式: 共有5679条查询结果,搜索用时 15 毫秒
121.
《Plant Production Science》2013,16(3):230-234
AbstractTo elucidate the possible participation of hemicellulose decomposition in lodging resistance, we studied the change of hemicellulose and cellulose content in the stems of rice during the ripening stage by methylation analysis and the expression of related genes by Northern blotting. In the rice stem in ripening stage, content of (1-3,1-4)-β-glucan, a component of hemicellulose, decreased markedly although the content of arabinoxylan, a major component of hemicellulose, and cellulose showed little change during the same growth period. On the other hand, expression of the Gns 1 gene, which may encode (1-3,1-4) -β-glucanase that catalyzes the degradation of (1-3,14) -β-glucan, increased sharply in the stem. The mechanism of decomposition of (1-3,1-4) -β-glucan in rice stem and the possible association with lodging resistance is discussed. 相似文献
122.
用灰色系统理论的斜率关联度分析法,求得影响黑杨派南方型无性系高生长的主要气候因子依次为:降水量、极低温、极高温、蒸发量、日照时数、积温、年均温(或相至湿度)、相以湿度(或年均温)、无霜期。 相似文献
123.
124.
AIM: To investigate the effect of intraventricular pressure change by volume overload (VOL) on expression of proto-oncogene c-fos,c-jun,c-myc and egr-1. METHODS: Left ventricular systolic pressure(LVSP) and left ventricular end diastolic pressure (LVEDP) of rat with VOL induced by aortacaval fistula operation and rats of control group were measured at 30 min, 1, 4, 6, 12 and 48 h after the operation,these mRNAs at the foregoing time points were measured by slot bloting method and quantified with densitometry. RESULTS: Be compared with the control group, VOL rats LVSP decreased (P<0.05) and declined most remarkably at 48 h,LVEDP elevated significantly at 30 min (P<0.05), reached a maximal value at 12 h and the levels of control group at 48 h (P>0.05) after the operation.The proto-oncogene expression signals were not detected in the control,negative controls and VOL rats at 30 min after the operation. The c-fos,c-jun and egr-1 mRNA signals appeared earlier,at 1 h, and c-myc mRNA increased later at 4 h.All reached peak value at 4 h and then declined gradually.The c-fos mRNA were not detected at 48 h. The c-myc,c-0jun and egr-1 mRNA persisted throught the entire observation period from 1 h to 48 h. CONCLUSIONS: During VOL early phase the overload have effect on expression of the proto-oncogene mRNA,c-fos,c-jun and egr-1 mRNA appear earlier, c-myc later,egr-1,c-jun and c-myc persist longer period, but the expressions do not strengthen with the ventricule wall load increase.This sequential induction pattern may reflect the time course regularity of the proto-oncogenes expression induced by VOL,and indicate the proto-oncogenes expression initiate while the heart load accumulate some extent and duration and the load magnitude may not play a critical role. 相似文献
125.
126.
Tradable biodiversity credit systems provide flexible means to resolve conflicts between development and conservation land-use
options for habitats occupied by threatened or endangered species. We describe an approach to incorporate the influence of
habitat fragmentation into the conservation value of tradable credits. Habitat fragmentation decreases gene flow, increases
rates of genetic drift and inbreeding, and increases probabilities of patch extinction. Importantly, tradable credit systems
will change the level of fragmentation over time for small and/or declining populations. We apply landscape equivalency analysis
(LEA), a generalizable, landscape-scale accounting system that assigns conservation value to habitat patches based on patch
contributions to abundance and genetic variance at landscape scales. By evaluating habitat trades using two models that vary
the relationship between dispersal behaviors and landscape patterns, we show that LEA provides a novel method for limiting
access to habitat at the landscape-scale, recognizing that the appropriate amount of migration needed to supplement patch
recruitment and to offset drift and inbreeding will vary as landscape pattern changes over time. We also found that decisions
based on probabilities of persistence alone would ignore changes in migration, genetic drift, and patch extinction that result
from habitat trades. The general principle of LEA is that habitat patches traded should make at least equivalent contributions
to rates of recruitment and migration estimated at a landscape scale. Traditional approaches for assessing the “take” and
“jeopardy” standards under the Endangered Species Act based on changes in abundance and probability of persistence may be
inadequate to prevent trades that increase fragmentation. 相似文献
127.
白菜型油菜双显性核不育896AB恢复系基因型的鉴定 总被引:9,自引:0,他引:9
以白菜型油菜双显性核不育896AB为材料,采用成对兄妹交和相应可育株自交,验证显性不育基因的遗传;用恢复系与全不育系测交,测交一代与临保系复交,验证显性恢复基因的抑制作用,并区分F2代育性分离为3:1和13:3的遗传类型。经4个年度的研究认为,育性是由一对显性不育基因MSMS和一对显性可育基因RfRf互作控制,且显性可育基 相似文献
128.
A subtractive cDNA library was developed to study genes associated with bud dormancy release in tree peonies.In order to identify genes that are highly expressed in buds released from dormancy,588 clones were examined by differential screening.Of these,185 clones were selected to be sequenced.A total of 37 unique sequences were obtained of which only 31 sequences have matches in the NCBI database or the Arabidopsis thaliana protein database.Semi-quantitative RT-PCR was used to confirm further the expression... 相似文献
129.
《Plant Production Science》2013,16(3):178-183
AbstractLeaf sheaths of rice plants are known to temporarily accumulate starch prior to heading, which is subsequently remobilized and transported into the panicle after heading. We investigated the time course for both carbohydrate content and steady state mRNA levels of enzymes related to starch and sucrose metabolism in the rice leaf sheath (Oryza sativa L. cv. Nipponbare). Leaf sheaths from the second leaf below the flag leaf accumulated high levels of starch before heading but they rapidly decreased after heading. In contrast, the flag leaf sheath did not accumulate as much starch. In the second leaf sheath, the mRNA levels of enzymes involved in starch synthesis, ADP glucose pyrophosphorylase (EC 2. 7. 7. 27), soluble starch synthase (EC 2. 4. 1.21) and branching enzyme (EC 2. 4. 1. 18) were high before heading, which coincided with rapid accumulation of starch. The mRNA levels of sucrose synthesis enzymes, cytosolic FBPase (EC 3. 1. 3. 11) and sucrose phosphate synthase (EC 2. 4. 1. 14), and the sucrose transporter (OsSUTI) increased at the time of heading, which was largely coincident with a decrease in the mRNA levels of starch synthesis enzymes. In the flag leaf sheaths, changes in mRNA levels of starch synthesis enzymes were not pronounced, however mRNA levels of sucrose synthesis enzymes and the sucrose transporter showed a clear increase throughout the heading period. The different characteristics observed between the two leaf sheaths will be discussed in relation to the sink to source transition. 相似文献
130.
The ABCB4 gene, also called MDR3, encodes the MDR3 protein which is localized to the hepatocyte canalicular membrane and is demonstrated to be a phosphatidylcholine translocase. The recent study showed that ABCB4 deficiency was associated with several cholestatic disorders, but the pathogenesis is not clear. This review highlights recent advances in the structure and function of ABCB4 gene, and the relationship between ABCB4 gene and cholestatic diseases. 相似文献